Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Critical But Overlapping Role of Fc?RIII and Fc?RIV in Activation of Murine Neutrophils by Immobilized Immune Complexes

doi:

Figure Lengend Snippet: Fc-receptor-blocking monoclonal antibodies used in this study

Article Snippet: Wherever possible, isotype controls binding to other neutrophil cell surface receptors were used, or the obtained results were confirmed by Fab preparation of the Fc-receptor-blocking mAbs (see Results ). table ft1 table-wrap mode="anchored" t5 caption a7 Clone Specificity Isotype Source RB6-8C5 Mouse Gr1 (Isotype Control) Rat IgG2b BD Biosciences 2.4G2 Mouse FcγRII/III Rat IgG2b BD Biosciences A19-3 − (Isotype Control) Hamster IgG1 BD Biosciences 9E9 Mouse FcγRIV Hamster IgG1 Jeffrey Ravetch 27-35 − (Isotype Control) Mouse IgG2b BD Biosciences IV.3 Human FcγRIIA Mouse IgG2b Jeanette Leusen HI30 Human CD45 (Isotype Control) Mouse IgG1 BD Biosciences 3G8 Human FcγRIII Mouse IgG1 BD Biosciences Open in a separate window caption a8 Fc-receptor-blocking monoclonal antibodies used in this study In experiments using Fc-receptor-blocking antibodies, neutrophils were preincubated with the indicated blocking antibodies or their isotype controls at 1 or 4 μg antibody per 10 6 murine or human cells, respectively.

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Critical But Overlapping Role of Fc?RIII and Fc?RIV in Activation of Murine Neutrophils by Immobilized Immune Complexes

doi:

Figure Lengend Snippet: A-B, Superoxide production of human neutrophils pretreated with an FcγRIII-blocking mAb or its isotype control (Iso Co) and plated on immune complex (IC)-covered surfaces. Panel A shows a representative experiment while panel B summarizes results from 8 independent experiments expressed in % of Iso Co. C-D, Gelatinase release (C) and spreading (D) of antibody-treated neutrophils plated on IC-covered surfaces. E, Respiratory burst of neutrophils triggered by 100 nM PMA. Unstimulated control values were subtracted in A, B and E. *, p < 0.05 compared to Iso Co.

Article Snippet: Wherever possible, isotype controls binding to other neutrophil cell surface receptors were used, or the obtained results were confirmed by Fab preparation of the Fc-receptor-blocking mAbs (see Results ). table ft1 table-wrap mode="anchored" t5 caption a7 Clone Specificity Isotype Source RB6-8C5 Mouse Gr1 (Isotype Control) Rat IgG2b BD Biosciences 2.4G2 Mouse FcγRII/III Rat IgG2b BD Biosciences A19-3 − (Isotype Control) Hamster IgG1 BD Biosciences 9E9 Mouse FcγRIV Hamster IgG1 Jeffrey Ravetch 27-35 − (Isotype Control) Mouse IgG2b BD Biosciences IV.3 Human FcγRIIA Mouse IgG2b Jeanette Leusen HI30 Human CD45 (Isotype Control) Mouse IgG1 BD Biosciences 3G8 Human FcγRIII Mouse IgG1 BD Biosciences Open in a separate window caption a8 Fc-receptor-blocking monoclonal antibodies used in this study In experiments using Fc-receptor-blocking antibodies, neutrophils were preincubated with the indicated blocking antibodies or their isotype controls at 1 or 4 μg antibody per 10 6 murine or human cells, respectively.

Techniques: Blocking Assay

Journal: Scientific Reports

Article Title: Antibody validation and scoring guidelines for ABCG2 immunohistochemical staining in formalin-fixed paraffin-embedded colon cancer tissue

doi: 10.1038/srep26997

Figure Lengend Snippet: The full membrane is displayed to reveal the presence of all reactive bands upon antibody incubation. The six different antibodies were applied to ABCG2 up-regulated (SN-38 RES) and parental LoVo, MDA-MB-231 and MCF7 cancer cell lines as indicated in the figure. The six different antibodies are applied in the six panels as follows: ( a ) mAb BXP-21, ( b ) mAb 6D171, ( c ) mAb 3G8, ( d ) pAb B7185, ( e ) pAb TA332085 and ( f ) pAb TA324234. β-actin (42 kDa) was used as a loading control. The molecular weight marker is indicated.

Article Snippet: The mouse mAb 3G8 was purchased from Abnova (Taipei City, Taiwan), and the immunogen was a recombinant protein corresponding to human ABCG2 (aa153-360).

Techniques: Membrane, Incubation, Control, Molecular Weight, Marker